Wide-field Two Photon Microscopy
نویسندگان
چکیده
منابع مشابه
Wide-field Time-correlated Single Photon Counting Microscopy
Time-correlated single photon counting (TCSPC) is a sensitive, robust and precise technique to measure luminescence decays.[1] For fluorescence microscopy, it is often implemented with beam scanning and single point detectors, but we have implemented a camera-based wide-field TCSPC method. We employ a photon counting image intensifier in combination with a 1 MHz frame rate CMOS camera, thus com...
متن کاملContinuous wave two-photon scanning near-field optical microscopy.
We have implemented continuous-wave two-photon excitation of near-UV absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 647-nm emission of an Ar-Kr mixed gas laser was used to excite the UV-absorbing DNA dyes DAPI, the bisbenzimidazole Hoechst 33342, and ethidium bromide in a shared aperture SNOM with uncoated fiber tips. Polytene chromosomes of Drosophila melanogast...
متن کاملUltra-large field-of-view two-photon microscopy.
We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of t...
متن کاملWide-field two-dimensional multifocal optical-resolution photoacoustic-computed microscopy.
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images optical absorption in tissue at high spatial resolution. To date, the majority of OR-PAM systems are based on single-focused optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens a...
متن کاملFast Scanning Two-Photon Microscopy
Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. The software controller was expanded so that random access scan in three dimensions could be hand...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optik & Photonik
سال: 2015
ISSN: 1863-1460
DOI: 10.1002/opph.201500035